Don Juan Fernández Pacheco, (20 December 1563, Escalona, Spain – 5 May 1615, Escalona, Spain), 5th Duke of Escalona, 5th Marquis of Villena and 5th Count of Xiquena was a Spanish nobleman and, between 1607 and 1610, viceroy of Sicily.
He was the son of Don Francisco Pacheco, 4th Duke of Escalona (1532-1574) and Juana Alvarez de Toledo.
He was Spanish ambassador in Rome between 1603 and 1606.
He was viceroy of Sicily between 1607 and 1610.
In 1589, he was made a knight in the Order of the Golden Fleece.
He married in 1594 with Serafina de Portugal Bragança (1566-1604), daughter of John I, 6th Duke of Braganza.
They had 10 children, of which 7 reached adulthood.